Hepcidin production is induced in neutrophils and macrophages by bacterial pathogens. (A) Immunofluorescence using anti-mouse hepcidin antibody on normal murine macrophages at baseline and upon exposure to Group A Streptococcus (GAS) or P aeruginosa (PA); magnification × 630. (B) Western blot analysis of hepcidin in murine macrophages and neutrophils at baseline and upon PA exposure. Addition of 10 μg blocking hepcidin peptide per milliliter of antibody was used to check for the specificity of the anti-mouse hepcidin antibody.