Hepcidin expression in spleen and liver of mice upon systemic infection. Immunohistochemistry using anti-mouse hepcidin antibodies on spleens (A) and livers (B) of mice 4 hours after intraperitoneal challenge with Pseudomonas aeruginosa or PBS control. (C) Real-time PCR for hepcidin-1 mRNA in liver and spleen of WT (CH3/HeouJ) and Tlr4^Lps-d (CH3/HeJ) mice challenged for 4 hours with PA. Data are shown as mean ± SD