Gene expression of CXCL7 in response to stromal signals. (A) CD14⁺ cells from 8 different healthy donors were cultured alone or cocultured with HS27a cells for 3 days. Nonadherent cells were harvested, and CXCL7 gene expression was determined by Syber Green real-time PCR and normalized to G3PDH gene expression. Data represent mean ± SE; P = .015 by Student t test. (B) CXCL7 gene expression in CD14⁺ cells from 10 healthy donors at day 0, day 5 in control media (RPMI with 10% FCS), and day 5 in culture in media conditioned by HS5 cells (HS5 CM). Data represent mean ± SE; P < .001 by Student t test comparing HS5 CM to either day 5 control or day 0.