Long-term survival of DC_VIPs in transplanted mice. Balb/c (H-2^d) mice received transplants of BMS from B6 (H-2^b) mice. CFSE-labeled DC_VIPs were injected 2 days after transplantation. (A) The presence of CFSE-labeled DC_VIPs in recipient spleens was determined at the indicated times by flow cytometry (n = 3). Inset: Histogram represents CFSE-labeling profile at day 5 after injection. (B) Ten days after injection, spleen CFSE-labeled DC_VIPs were sorted, stimulated with LPS (1 μg/mL) for 24 hours, and analyzed for CD11c, CD40, CD45RB, and CD80 expression by flow cytometry. Sorted CFSE-labeled DC_VIPs (10⁴ cells) were incubated with allogeneic T cells (5 × 10⁴) from B6 mice and proliferation was determined (n = 3). LPS-matured DCs (DC_controls) were used as controls.