Treatment with DC_VIPs does not abrogate cytotoxicity of transplanted T cells against leukemic cells. GVHD was induced in recipient Balb/c (H-2^d) mice by allogeneic transplantation of BMS from B6 (H-2^b) mice. Two days after transplantation, recipients were given injections of medium (none) or with BM-DCs from Balb/c (H-2^d) generated in the absence (DC_controls) or presence (DC_VIPs) of VIP. (A) Donor I-K^bCD8⁺ cells isolated from recipient spleens (untreated, •; DC_controls-treated, ○; DC_VIPs-treated, ▾) were subjected to cytotoxicity assays against leukemia A20 (H-2^d) and mastocytoma P815 (H-2^d) cells (n = 8). (B) Donor I-K^bCD8⁺ cells isolated from recipient spleens were analyzed for CD44 and CD62L expression by flow cytometry. Numbers represent the percentage of cells in each quadrant. Result is representative of 8 identical experiments.