Localization of PAI-2 in HEK-293 cells expressing ZNF198/FGFR1. (A) Analysis of the secretion of PAI-2 into the supernatants from cells transfected with GFP, GFP-tagged ZNF198 (ZNF), GFP-tagged ZNF198/FGFR1 (FUS), or GFP-tagged FGFR1 (after stimulation with bFGF) demonstrates that only those cells expressing ZNF198/FGFR1 secrete PAI-2. (B) Cell lysates from cells expressing the fusion kinase show that the cytoplasmic fraction contains the 47-kDa and 32-kDa forms of PAI-2 but not the nuclear fraction.