Kinetics of tether formation. (A-B) Washed blood-cell suspension (platelet count, 11 000/μL) perfused over immobilized dVWFA1 coated at low (A) or high (B) density (coating solution 1 or 20 μg/mL, respectively). Wall shear rates as shown. “Contact” indicates platelets interacting with the surface for at least 0.033 second; “translocation” specifies platelets moving on the surface by a distance more than 1 cell diameter in 2 seconds at lower than free-flow velocity; “tether” denotes platelets forming tethered DAPs. (C) Translocation velocity of platelets and their respective tethered DAPs. (D) Kinetics of tether formation at high dVWFA1 density and γw of 40 000 s–1. (E) Washed blood-cell suspension with reduced platelet count (11 000 platelets/μL) perfused over dVWFA1 at γw of 40 000 s–1. Isolated microparticles and tethers detached from platelets are seen while translocating on the surface. Scale bar = 5 μm. See Movie S7.
