MIST is dispensable for development of NK and NKT cells. (A) Cells isolated from liver (left) and spleen (right) of 4- to 6-week-old MIST-deficient mice (-/-) and their littermates (+/+) were stained with the indicated antibodies and analyzed by flow cytometry. The percentages of gated cells are shown. Data are representative of 4 independent experiments. (B) Expression of inhibitory receptors as well as activating receptors on NK1.1⁺TCRab^- NK cells from MIST-deficient mice. Histograms indicate expression of various inhibitory receptors (Ly receptors and CD94) and other activating receptors (NK1.1 and CD16) on NK cells in MIST-deficient (-/-) and wild-type NK cells (+/+). The gate used to calculate the percentage of cells expressing each receptor is shown by a horizontal line above each histogram. All histograms are representative of analyses from at least 3 mice of each genotype. (C) Flow cytometric analysis of knock-in EGFP fluorescence in spleen cells left unstimulated (arrows) or stimulated with α-GalCer (arrowheads) for 12 hours. FACS plot is shown for each set of markers, because there were no significant differences in phenotype between wild-type and MIST^+/Δneo mice. For each of the gated populations indicated, histograms for EGFP fluorescence were generated. Wild-type histograms (dotted lines) were overlaid with mutant histograms (solid lines).