MIST deficiency protects mice from lung metastases. (A) B16 melanoma cells and Colon-26 carcinoma cells were injected intravenously into syngeneic wild-type (□) and MIST-deficient mice (▪) on the C57BL/6 and BALB/c background (4 mice per group), respectively, and metastatic nodules were counted 14 days later. (B) Number of metastatic nodules formed by B16 melanoma cells in the lungs of wild-type and MIST-deficient mice treated with anti-asialo GM1 antibody to deplete NK cells, as described in “Materials and methods.” Statistical analysis was performed using the Student t test (^*P < .01; ^**P < .005). (C) Representative photographs of metastatic nodules in the lung are shown.