Involvement of IL-10 in the protective effect of DC_regs against lethality induced by CLP. Mice (5 animals/group) were given intraperitoneal (A) or intravenous (B) injections of mDCs or DC_regs (10⁶/mouse) 6 hours after CLP. ^*P < .01 compared with the untreated mice by the log-rank test. (C) Mice (5 animals/group) were given intraperitoneal injections of mDCs or DC_regs (10⁶/mouse) with or without control Ig (1 μg/mouse) or anti-IL-10 Ab (1 mg/mouse) 6 hours after CLP. ^*P < .01 compared with the untreated mice, †, compared with mice given injections of anti-IL-10Ab; ‡, compared with mice given injections of DC_regs plus anti-IL-10Ab, by the log-rank test. (D) Mice (5 animals/group) given intraperitoneal injections of mDCs or DC_regs (10⁶/mouse) obtained from normal mice or Il10KO mice 6 hours after CLP. ^*P < .01 compared with the untreated mice; †, compared with mice given injections of mDCs; ‡, compared with mice given injections of Il10KO mDCs; or §, compared with mice given injections of Il10KO DC_regs, by the log-rank test. Survival was monitored at the indicated times, and the results are representative of 4 experiments with similar results.