Figure 5.
![Figure 5. IL-15 but not IL-21 induces antiapoptotic effects and CLL B-cell proliferation. The antiapoptotic and mitogenic activities of IL-15 are counteracted by IL-21. (A) CD40-activated CLL B cells were cultured in triplicate wells of 96-well plates in the presence of different concentrations of rhIL-15 and/or rhIL-21 for 48 hours and then pulsed with 3H-thymidine (0.037 MBq/well [1 μCi/well]) for an additional 18 hours of culture. Data are expressed as mean ± SD of 3 repeated experiments. (B) Apoptosis was measured by DiOC6 staining of CLL B cells after 72 hours of culture in the presence, or absence of rhIL-21 and/or rhIL-15 (40 ng/mL). Horizontal bar M1 indicates fluorescence intensity corresponding to signals from viable cells.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/9/10.1182_blood-2005-09-3535/2/zh80090694950005.jpeg?Expires=1735825161&Signature=OREMTCe5J74n9BEfh01tivX1dASXEOwxydZR6l~KkKeaGWpuyTJdSCIFEkgpYGH9vwGTq~lXXzRUwm4-eYj6YaRLDNiBJHLN1E0S2Q8gB43U6sqRQDCBXghGp056lhaL95D5HBbA~Ubmvm1gOchFWo98mNTkypyXyNNYSR1Y51qJQwlrjNteVNau28NwVUHTkCNZ0ZVcEa7-9GYKX2aVlXT-5~2fM0J4FNrnTF70pYL2wHjDVBT8r3zLUQZcjPWNSQijIw1p8vvEe3PXdam~DyYubvj1nnXaUR3HOa00wxDeyaszpSaHMPdBsrJLrDb-oMwBIlFSw8gA79-ldyZVIA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IL-15 but not IL-21 induces antiapoptotic effects and CLL B-cell proliferation. The antiapoptotic and mitogenic activities of IL-15 are counteracted by IL-21. (A) CD40-activated CLL B cells were cultured in triplicate wells of 96-well plates in the presence of different concentrations of rhIL-15 and/or rhIL-21 for 48 hours and then pulsed with 3H-thymidine (0.037 MBq/well [1 μCi/well]) for an additional 18 hours of culture. Data are expressed as mean ± SD of 3 repeated experiments. (B) Apoptosis was measured by DiOC6 staining of CLL B cells after 72 hours of culture in the presence, or absence of rhIL-21 and/or rhIL-15 (40 ng/mL). Horizontal bar M1 indicates fluorescence intensity corresponding to signals from viable cells.
