Lineage reconstitution of mutant cells in competition with wild-type cells. FACS profiles of BM, spleen, and thymus from hematopoietic chimeric mice, indicating whether mutant cells (Ly5.2) differentiate into myeloid (Gr1), B-cell (B220⁺), or T-cell populations (CD4 and CD8) by simultaneous staining with anti-Ly5.2 antibody. Ly5.2⁺Hoxb4^-/- cells of the thymus were gated and analyzed on distribution of CD4 and CD8 single- and double-positive populations (not available for Hoxb1-b9 mutant). (A) Hoxb4^-/- chimeras and (B) Hoxb1-b9 chimeras. A color version of this figure is available online as Supplemental Figure S3.