CD62 and TLR4 colocalize on the platelet membrane. (A) Thrombin-stimulated platelets were simultaneously incubated with mouse anti-human TLR4 and rabbit anti-human CD62 showing colocalization on the platelet membrane. (Inset) Complex composed of 2 gold-labeled anti-CD62 antibodies (10 nm) and 2 anti-TLR4 antibodies (6 nm). (B) Colocalization of TLR4 and CD62 demonstrated on another platelet. (C) Washed thrombin-stimulated platelets were simultaneously incubated with the control antibodies mouse IgG1 and rabbit IgG. Nonspecific binding was noted, and colocalization of the antibodies was not observed.