TLR4 is required for LPS binding to mouse platelets. C57BL/10ScSn (n = 9) and C57BL/10ScN (n = 8) mice were given intraperitoneal injections of O157LPS, and platelets were taken after 4 hours and fixed on glass slides, as described for human PRP. PRP from a C57BL/10ScSn wild-type mouse (A) incubated with anti-O157LPS antibody showed aggregates of platelets labeled with O157LPS. No labeling of the O157LPS antibody was detected on platelets from a C57BL/10ScN mouse (B).