FVIII, VWF, IgG, FV, and fibrinogen levels in ex vivo perfusion medium of human lungs. Double lung blocks retrieved from brain-dead donors were ventilated through an endotracheal tube and perfused for 2 hours (donor 4). Alternatively, the perfusion medium was supplemented with desmopressin acetate (donor 1 and 2) or PMA (donor 3) 60 minutes after the start of the perfusion. In a control experiment, erythrocytes in complete medium were perfused for 2 hours with no lung inserted in the system (dashed line). FVIII:Ag (A), FVIII:C (B), VWF (C), FV (D), fibrinogen (E) and IgG (F) were measured in the perfusion medium. To compare the variations of FVIII:Ag, FVIII:C, or VWF concentrations to those of FV, fibrinogen, or IgG, the values of each variable in a whole perfusion experiment were divided by the corresponding concentration measured at 15 minutes. A 2-sided Wilcoxon paired test was then used to compare FVIII:Ag, FVIII:C, or VWF ratios with FV, fibrinogen, or IgG ratios obtained at the corresponding time points in the same perfusion experiment; °P ≤ .06; *P ≤ .03 for each of the comparisons with FV, fibrinogen, or IgG.