American Society of Hematology

Figure 6.

$Figure 6. Onset of ICN1-induced leukemia in RAG2-deficient mice is accelerated by treatment with anti-CD3ϵ antibodies. Rag2-/- mice were reconstituted with Rag2-/- bone marrow transduced with mICN1-containing or empty retroviruses. Ten days after transplantation, mice received single intravenous injections of anti-CD3ϵ. Cell suspensions from different organs were stained for the expression of CD4 and CD8 markers, and FACS analysis was performed to determine EGFP+ and EGFP- population percentages in peripheral blood (PB; A) 3 weeks after BMT and in bone marrow (BM; C) and spleen (SPL; E) 11 weeks after BMT of mice that underwent reconstitution with mICN1 (top panels) or empty-transduced bone marrow (bottom panels). Percentages of CD4+CD8+ cells in the EGFP+ (B,D,F; R2, left panels) and EGFP- (B,D,F; R3, right panels) fractions from the same organs are indicated. Total yield of spleens is also included. Data are representative of at least 3 independent experiments.$

Onset of ICN1-induced leukemia in RAG2-deficient mice is accelerated by treatment with anti-CD3ϵ antibodies.Rag2^-/- mice were reconstituted with Rag2^-/- bone marrow transduced with mICN1-containing or empty retroviruses. Ten days after transplantation, mice received single intravenous injections of anti-CD3ϵ. Cell suspensions from different organs were stained for the expression of CD4 and CD8 markers, and FACS analysis was performed to determine EGFP⁺ and EGFP^- population percentages in peripheral blood (PB; A) 3 weeks after BMT and in bone marrow (BM; C) and spleen (SPL; E) 11 weeks after BMT of mice that underwent reconstitution with mICN1 (top panels) or empty-transduced bone marrow (bottom panels). Percentages of CD4⁺CD8⁺ cells in the EGFP⁺ (B,D,F; R2, left panels) and EGFP^- (B,D,F; R3, right panels) fractions from the same organs are indicated. Total yield of spleens is also included. Data are representative of at least 3 independent experiments.

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