Figure 3.
Procaspase-2 is present at the CD95 DISC of different human cell lines. (A) SKW6.4, HUT78, BJAB, Raji, CEM, Jurkat A3 cells were stimulated with LZ-CD95L. CD95 was immunoprecipitated from 3 × 107 cells using anti–APO-1 antibody and Protein-A Sepharose. Immunoprecipitates along with total cellular lysates were subjected to 12% PAAG gels and immunoblotted with anti–caspase-2 mAbs, anti–caspase-8 mAbs C15, anti-FADD mAbs, and anti-CD95 polyclonal antibodies C20. (B) SKW6.4, HUT78, BJAB, CEM, and Jurkat A3 cells were stimulated with LZ-CD95L, which was followed by caspase-2 immunoprecipitation.
