Increased Ag uptake of BM-DCs from ICSBP^–/– mice. BM-DCs of ICSBP^–/– and WT mice were cultured for 6 days in the presence of GM-CSF. (A) Cells were then incubated for 40 minutes with 1 mg/mL DX-FITC and then stained for CD11c and CD86 expression. Density plots represent the uptake of the DX and the relative expression of CD86 in a population gated on forward–side scatter properties and on CD11c positivity. Data are representative of 3 separate experiments. (B) qRT-PCR analysis of mannose receptor (Mrc1) transcript levels in BM-DCs at different times of culture. Total RNA was extracted from ICSBP^–/– and WT BM-DCs at the indicated time points and used to generate cDNA that was analyzed by qRT-PCR. The cDNA concentrations were normalized to β-actin expression. The fold changes of Mrc1 transcripts of WT (•) and ICSBP^–/– BM-DCs (○) are expressed relative to 0 days using the ΔΔC_T method (“Materials and methods”). Results are representative of 3 separate experiments.