BM-DCs from ICSBP–/– mice fail to induce a CHS response in vivo. BM-DCs from WT and ICSBP–/– mice cultured for 7 days were magnetically sorted with anti-CD11c Microbeads and then labeled with FITC. FITC-loaded ICSBP–/– (□) and WT (•) DCs (5 × 105 cells) were then injected in the footpad of WT recipients. Six days after transfer, mice were challenged on 1 ear with 10 μL FITC. Ear thickness was measured on both ears 24 to 72 hours after challenge. Specific swelling was calculated by subtracting the thickness of the unchallenged ear from that of the FITC-challenged ear in each individual animal. Control swelling (♦) represents mice subjected to FITC challenge but not immunized. Results are expressed as the mean ± SD of at least 3 mice per group. One out of 3 separate experiments is shown.
