Regulatory activity of ^HGFMo and ^HGFMo-differentiated T cells. Primary MLR cultures were performed with ^HGFMo and purified, CFSE-labeled CD4⁺CD25^- T cells in the presence or in the absence of either neutralizing antibodies to IL-10/TGF-β (A) (each at 10 μg/mL) or blocking antibodies to ILT3 (B) (10 μg/mL). The percentage of proliferating, CFSE^dimT cells is depicted on the y-axis. ^*P < .01 compared with MLR cultures performed in the absence of the blocking antibodies. T-cell proliferation to GM4-DCs both in the presence and in the absence of the blocking Abs to IL-10 (A) and ILT3 (B) is also shown. Error bars indicate mean and SD. (C-D) DC-primed T cells were used for RT-PCR and flow cytometry studies of FoxP3 expression. Freshly isolated CD4⁺CD25⁺ and CD4⁺CD25^- T cells served as positive and negative control for FoxP3 expression, respectively (Figure S3). One representative experiment of 4 with similar results is shown.