Figure 6.
Melatonin but not tryptophan inhibited p300 HAT activity. (A) RAW 264.7 cells transfected with FLAG-p300 were treated with LPS/IFNγ in the presence or absence of melatonin or tryptophan. Nuclear extracts were immunoprecipitated with an anti-FLAG M2 Gel, and FLAG-p300 was eluted with a FLAG peptide. HAT activity of p300 was measured. (B) FLAG-p300–transfected cells were treated with LPS/IFNγ for 8 hours. FLAG-p300 was isolated and treated with MT or Trp for 30 minutes and HAT activity was measured. Each bar denotes mean ± SEM of 3 experiments.
