CXCL13 expression in thymus and sera during MG. (A) Microarray corresponding to CXCL13 gene expression level in thymuses of adult controls (A ctrl), untreated MG patients (MG), and MG patients treated by corticotherapy (MG-t) compared with the RNA reference obtained for each array. The y-axis shows the log 2 ratio centered on zero. Each dot corresponds to one comparison to the RNA reference. (B) Amplification by real-time PCR of CXCL13 gene in thymic samples from newborn girl controls (N ctrl), A ctrl, MG, and MG-t patients. Each dot represents the mean value of 3 different determinations. (C) Determination by ELISA of CXCL13 level expression in thymic samples from N ctrl, A ctrl, MG, and MG-t patients. Each dot represents the mean value of duplicates. (D) Determination by ELISA of CXCL13 level expression in sera samples from A ctrl, MG, and MG-t patients. Each dot represents the mean value of duplicates. In panels A-D, the bar represents the median value and the P values were obtained by the nonparametric one-way analysis of variance (Kruskal-Wallis test). (E) Correlation of CXCL13 expression determined by ELISA in thymic extracts and sera from MG untreated patients. The R2 and P value were obtained by the nonparametric correlation test (Spearman test).