LEN-mediated enhancement of NKT effector function. (A) NKT cells expanded in the presence or absence of LEN, as in Figure 1A, were tested for the ability to secrete interferon-γ in response to unpulsed or α-GalCer–pulsed DCs, by intracellular cytokine secretion (ICS) assay. The right panel shows data from individual donors (n = 7). Horizontal bars represent mean percent NKT cells secreting interferon-γ. (B) TaqMan analysis showing changes in cytokine gene expression in NKT cells (expanded initially with α-GalCer–loaded DCs) and cultured overnight with unpulsed or α-GalCer–loaded DCs in the presence of absence of LEN. Data are representative of 2 separate experiments. (C) NKT cells expanded in the presence of DEX alone, or DEX + LEN, as in Figure 1D, were tested for the ability to secrete interferon-γ in response to unpulsed or α-GalCer–pulsed DCs, by ICS assay (n = 4). (D) NKT cells in freshly isolated PBMCs from healthy donors were tested for interferon-γ secretion in response to vehicle or α-GalCer–pulsed DCs, by ICS assay, as in panel A (n = 5).