Measurement of nitric oxide and prostacyclin in BKB2R^-/- mice. Mouse serum or plasma were collected from control (B6129SF2/J) or BKB2R^-/- mice as indicated in “Materials and methods” for measurement of nitrate prepared from nitric oxide (A) or the stable prostaglandin I₂ (prostacyclin) derivative, 6-keto-PGF_1α (C), respectively. The data are the mean plus or minus SEM of 8 or more individual samples from BKB2R^-/- and control mice. (B) BKB2R^-/- mice and their controls were treated with 0.5 mg/mL L-NAME in their drinking water or with water alone for 7 days. After the treatment period, the time to vessel occlusion was determined using the Rose Bengal model for mouse carotid artery thrombosis. The data presented are the mean plus or minus SEM of 8 or more experiments with mice simultaneously treated with each of the conditions shown. (D) BKB2R^-/- mice and their controls were treated with 50 μg/mL nimesulide in their drinking water or with water alone for 10 days. After the treatment period, the time to vessel occlusion was determined using the Rose Bengal model for mouse carotid artery thrombosis. The data presented are the mean plus or minus SEM of 5 or more experiments with mice simultaneously treated with each of the conditions shown.