Angiotensin AT2 receptor antigen expression in the kidney. (A) Western blot of mouse kidney lysates for the AT2R receptor antigen. Kidney lysates from control and BKB2R-/- mice were prepared as indicated in “Materials and methods.” Equal amounts of total kidney lysate (12.5 μg to 50 μg) were added to each lane of the SDS-PAGE and, after electrophoresis, the gel contents were electroblotted onto nitrocellulose. An immunoblot was performed with anti-AT2R antisera followed by an antirabbit antibody conjugated with horseradish peroxidase. The protein was detected by the ECL system followed by an autoradiogram. The figure is a representative immunoblot experiment. (B) Densitometer scans were performed on multiple immunoblots of electrophoresed total kidney lysates from control and BKB2R-/- mice. The relative intensity of the bands was determined. The figure represents the mean plus or minus SEM of relative intensity of the bands of 3 or more experiments at each concentration of protein added. The data shown for 10 μg total kidney protein are the mean of only 2 experiments.
