Protein expression levels of Sep and cadherin-1 in peritoneal macrophages from noninfected mice (N) and PLC^–/–Trypanosoma brucei brucei–infected mice 2 weeks (W2) and 12 weeks (W12) after infection. Total cells from peritoneal lavage were stained with FITC-labeled anti-F4/80 antibodies (A) and with PE-labeled anti-F4/80 antibodies (B-C). Intracellular Sep expression (A), determined using Zenon-PE labeled anti-Sep antibodies (solid lines). Cadherin-1 surface (B) and intracellular (C) expression, determined using FITC-labeled anti–cadherin-1 antibodies (solid lines). Expression was determined by direct immunofluorescence and flow cytometry analysis. Expression profiles represent the distribution of fluorescent cells in function of fluorescence intensity in the PE channel of the flow cytometer (A) and in the FITC channel (B-C) for gated F4/80-positive macrophages. The dashed line corresponds to the background profile of cells stained with Zenon-PE–labeled control antibodies (A) and FITC-labeled control antibodies (B-C). Numbers between brackets indicate the background-subtracted median fluorescence intensities. Results are shown for 1 of at least 3 representative independent mice for each condition. *Significantly higher than in noninfected and in early stage–infected mice (P < .05).