American Society of Hematology

Figure 2.

$Figure 2. Representative CEP phenotype evaluation by flow cytometry. Peripheral blood was processed by Ficoll to enrich for the mononuclear cell fraction including CEPs. Panels in the top row show the gate used to exclude platelets, dead cells, and debris (left panel), the negative control (middle panel), and the gate made on CD133+ cells, regardless of CD45 expression (right panel). Panels on the bottom show CD133 expression with an irrelevant PE-labeled antibody (left panel), and the frequencies of CD133+ VEGFR2+ (middle panel) and CD133+ P1H12+ (right panel) EPCs. Antibodies used were PE-labeled anti-VEGFR2, PE-labeled P1H12 (CD146),4,12 and APC-labeled anti-CD133.$

Representative CEP phenotype evaluation by flow cytometry. Peripheral blood was processed by Ficoll to enrich for the mononuclear cell fraction including CEPs. Panels in the top row show the gate used to exclude platelets, dead cells, and debris (left panel), the negative control (middle panel), and the gate made on CD133⁺ cells, regardless of CD45 expression (right panel). Panels on the bottom show CD133 expression with an irrelevant PE-labeled antibody (left panel), and the frequencies of CD133⁺ VEGFR2⁺ (middle panel) and CD133⁺ P1H12⁺ (right panel) EPCs. Antibodies used were PE-labeled anti-VEGFR2, PE-labeled P1H12 (CD146),4,12 and APC-labeled anti-CD133.

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