Cytokine production following in vitro stimulation of T cells isolated from hemophilia A mice. Four animals per group were untreated, hFVIII plasmidtreated, or hFVIII plasmid-treated with immunomodulation by combination of Ctla4 and MR1 (4 animals from the group shown in Figure 3E,F). Splenic T cells were isolated from hemophilia A mice and incubated with hFVIII for 6 days. Production of IL-2, INF-γ, and IL-10 was measured from the culture media using ELISA.