Bone marrow cultures under myeloid growth conditions. (A) Myeloid colonies from young mice. Cells from wild-type (n = 6), Mll-AF4 (n = 7), and Mll-AF9 (n = 12) mice were plated in methylcellulose medium under myeloid conditions for 3 generations. (B) Multiparameter FACS analysis on pooled cells from the methylcellulose culture of third-generation cells grown under myeloid conditions. (C) Colony distribution of murine bone marrow cultures under myeloid growth conditions. The frequency of myeloid type I, II, and III colonies was determined from young wild-type, Mll-AF4, and Mll-AF9 mice for 3 generations. The error bars represent SEMs.