Cytoplasmic accumulation of WT-HNE in cells after ER stress induction. Subcellular localization of WT-HNE protein in HeLa cells with or without ER stress induction by tunicamycin (Tm). Cells were labeled with anti-HNE (green) and anticalnexin (ER marker; red) or antigiantin (Golgi marker; red). TO-PRO-3 was used to counterstain the nuclei (blue). Yellow represents colocalization of HNE protein with the organelle-specific markers.