Figure 1.
Inhibition of human myeloid progenitor growth by IL-17 family members. CFU-GM colony formation assays comparing all available IL-17 family proteins at varying concentrations were performed as described.5 IL-17 family cDNAs were isolated by reverse transcriptase–polymerase chain reaction (RT-PCR), subcloned into pCDNA3.1, and mature protein was purified to silver band homogeneity from 293 cell supernatants using nickel affinity columns.1 Control represents treatment with a similar quantity of protein isolation buffer. *P < .005.