American Society of Hematology

Figure 1.

$Figure 1. Purification and characterization of the 1F5 (scFv)4SA fusion protein. SDS-PAGE analysis of unpurified and iminobiotin-purified 1F5 (scFv)4SA with Coomassie Blue staining. The molecular weights in kilodaltons for Mark12 standards (Invitrogen) are indicated on the left (lane 1). Lane 2 contains a crude lysate from a culture of E coli-producing 1F5 (scFv)4SA. Lanes 3 and 4 contain the flow-through and wash fractions, respectively, from an iminobiotin purification column. Lanes 5 and 6 show the iminobiotin-purified FP either without boiling (lane 5) or denatured by boiling for 5 minutes (lane 6). The FP mass (174 kDa) and the monomer (44 kDa) are indicated on the right.$

Purification and characterization of the 1F5 (scFv)₄SA fusion protein. SDS-PAGE analysis of unpurified and iminobiotin-purified 1F5 (scFv)₄SA with Coomassie Blue staining. The molecular weights in kilodaltons for Mark12 standards (Invitrogen) are indicated on the left (lane 1). Lane 2 contains a crude lysate from a culture of E coli-producing 1F5 (scFv)₄SA. Lanes 3 and 4 contain the flow-through and wash fractions, respectively, from an iminobiotin purification column. Lanes 5 and 6 show the iminobiotin-purified FP either without boiling (lane 5) or denatured by boiling for 5 minutes (lane 6). The FP mass (174 kDa) and the monomer (44 kDa) are indicated on the right.

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