Figure 1.
Validation of quantitative RT-PCR for FcγRII isoforms on the LightCycler. The products obtained in PCR were separated on 1% agarose gel. GUS, FcγRIIb2, and then FcγRIIa were loaded. The first 3 slots are the serial dilutions of the standard curve, followed by the nontemplate control, followed by duplicates of 2 samples.