SA anti-CD28 treatment induces increased human thymic output despite T-cell depletion and high T-cell turnover in the periphery of HIS (BALB-Rag/γ) mice. (A) The percentage of CD3⁺ T cells among CD45⁺ cells (top) and the absolute number of human T cells (bottom) was monitored in the blood of IgG1 control (▪) and SA anti-CD28-treated mice (○) over a 2-month period (mean ± SEM). Representative results from 1 experiment out of 3 are shown. (B) Control wild-type BALB/c mice (m) and HIS (BALB-Rag/γ) mice (H) were treated for 1 day with BrdU and incorporation by cells of the indicated subsets was analyzed (top panel). Proportion of Annexin-V⁺ cells was also determined in the indicated populations (bottom panel). Representative results from 1 experiment out of 2 are shown. Horizontal bars indicate mean values.