Effect of κCHEB LC conditional deletion on renal morphology. (A-B) κ-CHEB/κ-del mouse, left nephrectomy, performed before pI-pC-induced VκJκ CHEB deletion. (A) Light microscopy, araldite inclusion, toluidine blue staining, oil immersion (original magnification × 1000). (B) Electron microscopy (original magnification × 5000). Numerous crystalline inclusions within the cytoplasm of proximal tubular cells (arrows). (C-D). Same κ-CHEB/κ-del mouse, right nephrectomy, performed 2 months after pI-pC–induced VκJκ CHEB deletion. (C) Light microscopy, araldite inclusion, toluidine blue staining, oil immersion (original magnification × 1000). No more crystalline inclusions were observed in proximal tubular cells. By electron microscopy (panel D, original magnification × 5000), only few and small crystals were detected in phagolysosomes of proximal tubular cells (arrow).