Viral gene expression in spleen and bone marrow cells recovered from NOD/SCID-hu mice reconstituted with rKSHV.219-infected CD34⁺HPCs. Bone marrow cells recovered from rKSHV.219 mouse D6 were cultured ex vivo for 33 days in complete IMDM. Bone marrow cells were analyzed by IFA using a rat anti-LANA-1 primary antibody and an AMCA antirat secondary antibody. Bone marrow cells recovered from a NOD/SCID mouse inoculated with heat-inactivated rKSHV.219-infected HPCs also were analyzed. BCBL-1, a KSHV-positive primary effusion lymphoma cell line, was used as a positive control for LANA-1 IFA. U937, a myeloid leukemia cell line negative for KSHV infection, was used as a negative control. Cells were visualized under a 20 ×.0.45 Plan-Fluor extra-long working distance (ELWD) dark medium (DM) objective lens (Nikon, Tokyo, Japan); total magnification, ×/200.