Figure 2.
Figure 2. Formation of canine FVIII-neutralizing antibodies in hemophilia A mice. (A) Correlation between the overexpression of canine FVIII and subsequent presence of FVIII-neutralizing antibodies in mice in which FVIII activities were detectable before the onset of FVIII-neutralizing antibodies. The FVIII activities at weeks 2 and 6 after vector injection were determined by Coatest. The FVIII-neutralizing antibodies at weeks 2 and 6 were determined by Bethesda assay. (B) Lack of correlation between the levels of FVIII-neutralizing antibodies and transgene DNA in liver in individual mice. The copy numbers of canine FVIII DNA per diploid genome in mouse liver were quantified by Southern blotting analysis.

Formation of canine FVIII-neutralizing antibodies in hemophilia A mice. (A) Correlation between the overexpression of canine FVIII and subsequent presence of FVIII-neutralizing antibodies in mice in which FVIII activities were detectable before the onset of FVIII-neutralizing antibodies. The FVIII activities at weeks 2 and 6 after vector injection were determined by Coatest. The FVIII-neutralizing antibodies at weeks 2 and 6 were determined by Bethesda assay. (B) Lack of correlation between the levels of FVIII-neutralizing antibodies and transgene DNA in liver in individual mice. The copy numbers of canine FVIII DNA per diploid genome in mouse liver were quantified by Southern blotting analysis.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal