Effect of imatinib or LY294002 on BCRP expression and Akt expression or phosphorylation. (A-B) Effects on BCRP expression. K562/BCRP (A) and K562/BCRP-MX10 cells (B) were exposed to LY294002 or imatinib for 14 hours at 37°C. Total cell lysates were extracted and then 40 μg of total cell lysates was subjected to Western blotting. These Western blots represent 3 individual experiments. (C) Effects on Akt expression and phosphorylation. K562/BCRP-MX10 cells were exposed to imatinib or LY294002 for 14 hours at 37°C. Total cell lysates were then extracted and subjected to Western blotting for Akt or phospho-Akt (S473) protein. Forty micrograms of cell lysate was loaded onto each lane. These Western blots are representative of 3 individual experiments done on different days.