Figure 5.
Co-infusion of allogeneic T cells increases the engraftment of myeloid dendritic cells after CD34+ cell transplantation in NOD/SCID mice. Engraftment of human myeloid DCs was compared in the marrow of NOD/SCID mice 6 weeks after cotransplantation of CB CD34+ cells with allogeneic (allo) blood T cells (n = 5) or autologous (auto) CB T cells (n = 4). All mice showed evidence of huCD45+ cells, which was not significantly different between mice also receiving allogeneic versus autologous T cells. More CD19-BDCA1+ myeloid dendritic cells (A) were observed in huCD45+ cells from mice that received cotransplants of allogeneic blood T cells (Allo) or autologous CB T cells (Auto). A trend toward more CD1a+ immature DCs (B) was also detected. Dots represent individual animals; bars, mean values.