DC viability in response to treatment with bortezomib. (A) DCs generated from adherent monocytes by culture in the presence of GM-CSF and IL-4 were harvested at day 6 of culture and treated for 24 hours with the indicated concentrations of bortezomib. Thereafter, cells were harvested and stained with 5 μg/mL PI, and PI^neg (viable) DCs were enumerated by flow cytometry. Means of triplicates with SDs are presented. *P < .05 (B) Adherent monocyte-derived DCs were cultured in the presence of GM-CSF and IL-4 for 6 days. Thereafter, cells were stimulated for 24 hours with or without TNF-α (20 ng/mL) or LPS (200 ng/mL) in the presence or absence of 5 ng/mL bortezomib. Cells were subsequently harvested, stained with PI, and analyzed by flow cytometry. Results are shown as means of triplicates with SDs.