LPS-induced DC migration toward CCL19/MIP-3β is impaired by bortezomib. DCs were generated by incubating adherent monocytes with GM-CSF and IL-4. At day 6 of culture, cells were exposed for 24 hours to bortezomib at the indicated concentrations (in ng/mL). LPS (100 ng/mL) was added as a stimulus to the culture medium for the last 16 hours. DCs were then harvested and washed, and migration toward CCL19 was analyzed using transwell chambers. DCs (2 × 105) were seeded in the upper chamber in triplicates, and the number of migrated DCs was analyzed after 16 hours. (B) CCR7 expression was detected by flow cytometry on DCs generated from adherent monocytes as described in panel A. Cells were treated with or without bortezomib (1 ng/mL) for 24 hours and exposed to LPS (100 ng/mL) during the last 16 hours.
