Cotreatment with LBH589 and AMN107 causes greater attenuation of p-AKT, p-STAT5, p-CrkL, Bcl-xL, and c-Myc but induces more p27, Bim, and PARP cleavage in K562 cells. (A) Following treatment of K562 cells with 20 nM LBH589 and/or 50 nM AMN107 for 24 hours, Western blot analyses of Bcr-Abl, p21, and p27 was performed on the cell lysates. The levels of β-actin served as the loading control. (B) Following treatment of K562 or LAMA-84 cells with 20 nM LBH589 and/or 50 nM AMN107 for 24 hours, Western blot analyses of p-STAT5, STAT5, p-CrkL, Bcl-xL, c-Myc, Bim, and PARP was performed on the cell lysates. The levels of β-actin served as the loading control.