Figure 2.
Figure 2. In vitro characterization of α2A–/–-deficient platelets. (A) Heparinized PRP was stimulated with ADP (5 μM) and U46619 (1 μM or subthreshold concentration of 0.1 μM) in the presence or absence of 10 μM epinephrine (Epin), and light transmission was recorded on a Fibrintimer 4-channel aggregometer. Results shown are representative of 6 individual experiments. (B) Washed whole blood from wild-type or α2A–/– mice was incubated with 5 μM ADP and 1 μM U46619 (U46) in the presence or absence of 10 μM epinephrine (Epin) for 15 minutes at room temperature. Activation of αIIbβ3 and P-selectin expression was analyzed by flow cytometry. Results are presented as mean ± SD of 5 mice per group.

In vitro characterization of α2A–/–-deficient platelets. (A) Heparinized PRP was stimulated with ADP (5 μM) and U46619 (1 μM or subthreshold concentration of 0.1 μM) in the presence or absence of 10 μM epinephrine (Epin), and light transmission was recorded on a Fibrintimer 4-channel aggregometer. Results shown are representative of 6 individual experiments. (B) Washed whole blood from wild-type or α2A–/– mice was incubated with 5 μM ADP and 1 μM U46619 (U46) in the presence or absence of 10 μM epinephrine (Epin) for 15 minutes at room temperature. Activation of αIIbβ3 and P-selectin expression was analyzed by flow cytometry. Results are presented as mean ± SD of 5 mice per group.

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