American Society of Hematology

Figure 4.

$Figure 4. Bak coimmunoprecipitates with Bcl-xL in lysates from FDC-P1 cells, wild-type, and Bad-/- ;Bim-/- FDM cells cultured in the presence of IL-3 but not in lysates from cells deprived of IL-3. Bak-Bcl-xL association is retained in IL-3-deprived Puma-/- FDM cells. (A) Anti-Bak and anti-Bax Western blots of anti-Bcl-xL immunoprecipitated (IP) material from the heavy membrane fraction of FDC-P1 cells cultured in the presence (0) or absence of IL-3 for 10 and 16 hours. Anti-Bcl-xL immunoprecipitates, as well as the total input and the supernatant fraction after each immunoprecipitation (SN fraction) are shown. LC indicates immunoglobulin light chain of antibody used for immunoprecipitation. (B) Anti-Bak Western blots of anti-Bcl-xL immunoprecipitated from the heavy membrane fraction of wild-type, Bad-/-;Bim-/-, and Puma-/- FDM cells cultured in the presence (0) or absence of IL-3 for 12 and 24 hours.$

Bak coimmunoprecipitates with Bcl-xL in lysates from FDC-P1 cells, wild-type, and Bad^-/-;Bim^-/- FDM cells cultured in the presence of IL-3 but not in lysates from cells deprived of IL-3. Bak-Bcl-xL association is retained in IL-3-deprived Puma^-/- FDM cells. (A) Anti-Bak and anti-Bax Western blots of anti-Bcl-xL immunoprecipitated (IP) material from the heavy membrane fraction of FDC-P1 cells cultured in the presence (0) or absence of IL-3 for 10 and 16 hours. Anti-Bcl-xL immunoprecipitates, as well as the total input and the supernatant fraction after each immunoprecipitation (SN fraction) are shown. LC indicates immunoglobulin light chain of antibody used for immunoprecipitation. (B) Anti-Bak Western blots of anti-Bcl-xL immunoprecipitated from the heavy membrane fraction of wild-type, Bad^-/-;Bim^-/-, and Puma^-/- FDM cells cultured in the presence (0) or absence of IL-3 for 12 and 24 hours.

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