Figure 5.
Increased levels of soluble, enzymatically active VAP-1 in cord blood. (A) The levels of soluble VAP-1 protein (sVAP-1; mean ± SEM) in adult and cord blood were determined using a sandwich ELISA. (B) The SSAO activity of sVAP-1 (mean ± SEM) in sera was determined using the fluorometric assay. (C) The concentration of sVAP-1 was correlated to the SSAO activity in the 12 cord blood samples. (D) VAP-1 is the major SSAO enzyme in cord blood. Serum samples were left untreated or immunodepleted with anti-VAP-1 or control mAb beads, and the remaining SSAO activity (mean ± SEM; n = 3) was determined. **P < .01 between the control mAb and anti-VAP-1 mAb-depleted samples.