Epo profiles obtained by double immunoblotting. (A) Acceptance criteria for rhEpo, as defined by WADA.1 (B) Figure 1A from Beullens et al.2 Lane 1, epoetin β; lane 2, darbepoietin α; lane 3, “false-positive” urine sample collected immediately after exercise; lane 4, negative urine sample collected 1 hour after the sample in lane 3. (C) Figure 1 from Lasne. Lane 5, mixture of epoetin β and darbepoietin α; lane 6, urine sample after strenuous exercise; lane 7, natural urinary Epo; lane 8, urine sample showing protein P, unrelated to Epo; lane 9, urine sample in case of epoetin administration. Bands in panels B and C are numbered according to the criteria defined in panel A. Using the acceptance criteria of panel A, lanes 3 (panel B), 6, and 9 (panel C) should be considered as an adverse analytical finding corresponding to the presence of rhEpo.
