Figure 1.
Figure 1. Typical colonies observed in ET and in RARS with ET features. Colonies are shown after May-Grünwald-Giemsa staining of collagen gels and are typical of ET (A-C) or of RARS with ET features (D-E). (A) Endogenous megakaryocytic colony, grown without serum or cytokine. (B,D) Erythroid colonies grown in the presence of cytokines; note the small size of the RARS colony (D). (C) Detail of erythroblasts of the colony shown in panel B. (E) Detail of erythroblasts of the colony shown in panel D; note extensive cell death. Images were obtained using a Leica microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 10×/0.80 numeric aperture (NA) (A,B,D) or a 100×/0.80 NA (C,E) objective. Images were acquired using a Sony Power HAD DXC-950P camera (Sony, Tokyo, Japan) and Tribun ICS/Thunder 17 acquisition software. Images were processed using Microsoft PowerPoint 2000 (Microsoft, Redmond, WA).

Typical colonies observed in ET and in RARS with ET features. Colonies are shown after May-Grünwald-Giemsa staining of collagen gels and are typical of ET (A-C) or of RARS with ET features (D-E). (A) Endogenous megakaryocytic colony, grown without serum or cytokine. (B,D) Erythroid colonies grown in the presence of cytokines; note the small size of the RARS colony (D). (C) Detail of erythroblasts of the colony shown in panel B. (E) Detail of erythroblasts of the colony shown in panel D; note extensive cell death. Images were obtained using a Leica microscope (Leica Microsystems, Wetzlar, Germany) equipped with a 10×/0.80 numeric aperture (NA) (A,B,D) or a 100×/0.80 NA (C,E) objective. Images were acquired using a Sony Power HAD DXC-950P camera (Sony, Tokyo, Japan) and Tribun ICS/Thunder 17 acquisition software. Images were processed using Microsoft PowerPoint 2000 (Microsoft, Redmond, WA).

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