Long-term cultures expressing CM exhibit a myelomonocytic phenotype with eosinophilia. (A) Long-term cultures expressing CM or AE, and leukemia cell lines from patients with inv(16) (ME-1) or t(8;21) (Kasumi-1) were analyzed by Western blot using an anti-CBFβ antibody. (B) Surface marker expression of long-term CM cultures expressed as averages of 4 independent cultures with SDs. (C) Photographs of typical cultures transduced with MIG or CM and grown for 8 weeks. (D) Wright-Giemsa-stained cytospins of week 10 MIG and CM cultures, with eosinophils indicated by the arrows. (E) Average eosinophil counts determined by Wright-Giemsa staining of 4 independent MIG- and CM-matched cultures (5-10 weeks in culture) and 3 independent longer-term (week 15-20) CM cultures. Error bars represent SD.