Phenotypic analysis of ES-derived cells before and after EB differentiation. Single cell suspensions were prepared from undifferentiated ES cells, from disassociated D14EBs, from nonadherent cells, or from expanded nonadherent cells. Nonadherent cells (NA cells) and expanded nonadherent cells (Exp NA cells) were derived in medium supplemented with 100 ng/mL Flt3-L. Surface marker expression was analyzed by flow cytometry. Error bars indicate SEM. § indicates that the marker expression in the cells of D14EBs was significantly different from that of undifferentiated ES cells (P < .05). ^* indicates that the marker expression in the nonadherent cells was significantly different from that in cells of D14EBs (P < .05). # indicates that the marker expression in the expanded nonadherent cells was significantly different from that in the nonadherent cells prior to expansion (P < .05).