Low Flt3-L and SF enhance the differentiation of erythroid cells. (A) Nonadherent cells derived from intact D14EBs prior to (NA cells) or after (Exp NA cells) expansion were analyzed for the expression of glycophorin-A. (B) Dissociated D14EBs were expanded for 8 days in GEM supplemented with 20 ng/mL of Flt3-L (GEM-low Flt3-L) or in the SF medium without FBS (SF) or with 15% FBS (SF + FBS) and analyzed for the expression of CD45 and glycophorin-A. (C) Nonadherent cells generated from intact D14EBs were expanded for 8 days in suspensions in GEM-low Flt3-L or in the SF medium and examined for the expression of glycophorin-A and CD45. (D) The erythroid colony formation by expanded nonadherent cells as described in panel C. (E) Dissociated D7EBs were expanded for 8 days in the SF medium and analyzed for the expression of CD45 and glycophorin-A. ^* indicates that the 2 sets of data are significantly different (P < .05). (A,D) Error bars indicate SEM.